ЦИАНОКОБАЛАМИН-СВЯЗЫВАЮЩИЙ БЕЛОК ТЕРМОФИЛЬНОГО МИКРООРГАНИЗМА

Сергей Юрьевич Симоненко,Иван Владимирович Артамонов,Виктор Сергеевич Лактюшкин,Никита Александрович Кульдюшев

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ЦИАНОКОБАЛАМИН-СВЯЗЫВАЮЩИЙ БЕЛОК ТЕРМОФИЛЬНОГО МИКРООРГАНИЗМА

С. Ю. Симоненко,

И. В. Артамонов,

В. С. Лактюшкин,

Н. А. Кульдюшев

2025-05-05

https://doi.org/10.24108/preprints-3113548

Генетические технологии, такие как редактирование генома или репрограммирование клеток с помощью вирусов, изменяют последовательности ДНК. Витамин B12 участвует в метаболизме фолата и SAM, которые необходимы для репликации, синтеза нуклеотидов, восстановления и метилирования ДНК и гистонов, а значит его дефицит может снизить эффективность и безопасность применения генетических технологий. В геномах термофильных микроорганизмов, собранных на территории Российской Федерации, нами был найден и клонирован ген периплазматического B12-связывающего белка термофильной бактерии Fervidobacterium riparium. Рекомбинантный белок обладает высокой термостабильностью, а также связывается с витамином B12 по результатам абсорбционной спектрофотомерии и дифференциальной сканирующей калориметрии. На данный момент не существует чувствительных неинвазивных методик для динамического определения внутриклеточных концентраций B12, но охарактеризованный нами белок мог бы стать сенсорным доменом генетически кодируемого флуоресцентного биосенсора B12 в перспективе заменив неоптимальные подобные биосенсоры и предоставив новый молекулярный инструмент, в том числе для оптимизации применения генетических технологий.

Ссылка для цитирования:

Симоненко С. Ю., Артамонов И. В., Лактюшкин В. С., Кульдюшев Н. А. 2025. ЦИАНОКОБАЛАМИН-СВЯЗЫВАЮЩИЙ БЕЛОК ТЕРМОФИЛЬНОГО МИКРООРГАНИЗМА. PREPRINTS.RU. https://doi.org/10.24108/preprints-3113548

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